We studied micelles of PS197-b-PAA47 loaded with thiocyanomethylthiobenzothiazole (TCMTB) biocide. Crew-cut micelles of PS197-b-PAA47 are prepared in dioxane by slow water addition. The micelles size is 26 nm by TEM and 50 nm by DLS. The micelles are loaded afterwards with the hydrophobic antibacterial agent – TCMTB. The efficiency of micelles loading is measured by the UV-vis technique. Maximum loading of biocide in micelles is around 30 wt%. Loaded micelles are exposed to bacteria (E.coli) and their deactivation is evaluated. SEM shows that the micelles adsorb on the surface of the bacteria; also, distinct differences are seen between bacteria treated with empty micelles and those treated with biocide-loaded micelles. Deactivation of the bacteria by TCMTB biocide is studied by exposing E-coli BL-21 bacteria to the loaded micelles. The deactivation is measured using a UV-vis technique. The absorbance of bacteria deactivated by loaded micelles is compared to the absorbance of bacteria in water and to those exposed to solutions containing empty micelles. It was found that E-coli bacteria do not grow with time in the presence of TCMTB loaded micelles; they are deactivated. Empty micelles of PS197-b-PAA47 do not affect the growth of the E-coli bacteria; the bacteria behave similarly to those exposed to a food-water solution.