We present an update of a modified atomic force microscopy (AFM) technique for the measurement of adhesion forces between synthetic polymers and bioparticulates implicated in asthma and indoor air pollution. We believe this would allow us to better understand the mechanisms by which bioparticles are entrained in man-made carpeting and upholstery. Polymers employed in our study were Nylon 6, Nylon 6,6, polyamide 12 and polystyrene (as a control). We first describe the use of three previous models for interpreting AFM force-distance data and calculating work of adhesion. We also discuss a square-pyramid-flat-surface (SPFS) model framework developed for calculating Hamaker constants. We find that the SPFS model yields the most reliable estimates of contact radius. This model is applied to calculate Hamaker constants for bioparticle-polymer interactions: E. coli with those of ragweed (A. artemisiifolia) and the black mold (A. niger). Results of relative Hamaker constants between these three bioparticles and the four polymers will be discussed in terms of their chemical surface features.