The influence of temperature and solution chemistry on cell deposition in porous media has been investigated in a well-controlled packed-bed system using Escherichia coli D21g. Three different temperatures (4, 10, and 25^oC) and two different electrolytes (KCl and CaCl₂) were employed for the experiments. To compliment the transport experiments, the bacterial viability, cell size, hydrophobicity, elecrophoretic mobility, and extracellular polymeric substances (EPS) composition were characterized. These analyses indicated the cell surface chemistry's sensitivity to solution chemistry and temperature. Additionally, transport experiments using 1 μm carboxyl modified latex (CML) particles were conducted to compare with E. coli D21g deposition trends. The experimental results showed that the deposition rate of CML particles increased with temperature in the presence of both electrolyte, suggesting that enhanced Brownian motion and decreasing viscosity results in greater transfer of particles to the collector surface. On the other hand, the E. coli D21g experimental results indicated the deposition rate and attachment efficiency (α) of the cell were greatest at 10^oC in the presence of KCl; however, the impact of temperature was negligible in the presence of CaCl₂. Based upon these results, it has been observed that an additional biological phenomenon, which has not been involved in classical filtration theory (CFT), contributes to the deposition kinetics of E. coli D21g in addition to physicochemical mechanisms.