Abstract The virus model of T4 bacteriophages can be genetically engineered to possess copies of cellulose-binding modules (CBM) on the capsids. This allows them to bind specifically onto cellulose surfaces. In our study, the thin film of regenerated cellulose is fabricated from cellulose triacetate and used as the model substrate. The adhesion experiments are performed in an impinging jet apparatus, in which the bacteriophages are subjected to a stagnation point flow under well-defined hydrodynamic and physicochemical conditions. The quantitative information of deposition kinetics for such nano-scale particles is obtained by the evanescent wave light scattering technique. The experimental conditions such as flow rates, ionic strength and pH values are varied in order to eliminate non-specific adsorptions and to achieve the optimal conditions for CBD/T4-ellulose binding reaction. (Figure 1. SEM image of genetically modified CBM bacteriophages T4 (white dots) on regenerated cellulose film.)