While the protein molecules are located adjacent to solid surface, the interaction occurs between protein molecules and solid surface. These interactions include: Interaction of Hydrophobic surface, Ionic interaction, Hydrogen and Vander Waals interactions. The Hydrogenic interaction is of negligible amount and hence isn't discussed here. Ionic interaction is the cause of interaction between different particles and Vander Waals interaction is the cause of non aqueous solvent medium with solutes. In this research, the adsorption of lysozyme and albumin on the surface of 2-hydroxy ethyl methacrylate (HEMA) with copolymers with Acrylic Acid (AA) (99%weight HEMA+ 1% weight AA), support is measured in the solvents of water, ethanol and acetone at pH=7.2 by UV spectroscopy. In pH experimental, albumin and lysozyme have negative and positive surface charge respectively and (HEMA-AA) surface has negative charge due to carboxyl groups. The proteins adsorption in water solvent is more compared with acetone solvent. In the last case proteins adsorption equals zero. The main reason for this phenomenon is the discharge of proteins in acetone solvent.