We report a novel method to manipulate aqueous films of nanoscale thickness by electric field. The ultrathin liquid films with tens of nanometer thickness were formed on hydrophilic mica surfaces under saturation humidity conditions. Applying electric field engendered tangential flow in the film by DC electroosmosis. The direction and speed of the film flow, visualized with fluorescent markers, were readily controlled by the external DC electric field. The film flow rate was characterized as a function of ionic strength, pH of the fluid as well as external electric field. The resulting flow characteristics were consistent with the theory of electroosmosis. To manipulate the flows in a controlled geometry, we confined the flow in 2D “virtual wall” channels by selective hydrophobization with micro-contact printing. This novel 2D nanofluidics methodology will provide a basis for molecular transport and manipulation as well as an enhanced nano scale and molecular level understanding of the interaction of fluids with surfaces.