Simulated Moving Bed (SMB) processes have become the industry standard for many production-scale separation applications, and the technology continues to receive heightened attention in the fields of pharmaceuticals, bio-separations, and enantiomeric separations. Advances in the technology have also made it a very viable, cost-effective option for low relative volatility, heat-sensitive specialty materials that cannot be separated via traditional means such as distillation or evaporation. The Dow Chemical Company has developed process technology involving methanolysis, hydroformylation, and hydrogenation of vegetable oils to produce hydroxyl methyl esters as precursors to polyurethane and other value-adding products. Although the hydroformylation/hydrogenation reaction mixture can be used without separation to produce polyols suitable for flexible polyurethane foams, production of other specialty products such as rigid foams and cast parts requires isolation of various fractions rich in monols or diols or low in non-hydroxy components. To fully obtain maximum value from bio-based resources in the specialty product realm, a means of separating the various reaction components is needed. In this work, a simulated moving bed (SMB) chromatography miniplant was used to determine the viability of the technology for separating these components. The technology was shown to be a viable option for 1) separating a soy reaction mixture into a fraction with 95 % monol and another with > 99 % diol; 2) separating a soy reaction mixture with saturates removed into two fractions with > 95 % monol and > 99 % diol. All purities are on a monol/diol only basis.