> Chinese herbal

> therapies for centuries due to its bioactivities

> derived from the

> lanostanoid triterpenes ingredients. This paper

> introduces the superior

> supercritical fluid extractions (SFE) for these

> triterpenes for saving

> the consumption of time and organic solvents. The

> thrift of latter item

> in purification and separation will benefit the

> environment. The yield

> of

> lanosta-7,9(11),24-trien-3-one,26,27-dihydroxy-(9Cl)

> (GA), for an

> example, by SCF is 70% higher than that obtained by

> traditional organic

> solvent extractions. In consequence, a sufficient

> sample can be

> obtained for 13C and 1H NMR studies. The chemical

> shifts of triterpines

> were unambiguously assigned by varies 1D and 2D NMR

> spectroscopy. Both

> the fused ring structure and the side chain

> structure were elucidated

> from the 13C-1H correlation NMR spectra. The

> absolute configuration

> about the chiral carbon was determined by NOE NMR

> spectra. Thus, the

> structure and the absolute configurations of the

> chiral carbons of GA

> have been determined. The structural information of

> GA will facilitate

> the further development of drug design.

> (Acknowledgement to the

> High-Field Biomacromolecular NMR Core Facility

> supported by the National

> Research Program for Genomic Medicine and Academia

> Sinica, Taiwan)