Nucleation and Stability of Nanotubes from DNA Tiles
DNA self-assembling systems are poised to play a major role in nanotechnology. One particularly versatile approach consists of building small DNA structures called tiles, which can be programmed via single stranded overhangs (i.e. sticky ends) to assemble into larger structures (e.g., 2D crystals) with well-defined architectures. Little is known, however, about the kinetics of the assembly process and how it might be controlled via sequence design to yield larger or more perfect assemblies. We measured kinetic and thermodynamic parameters of DNA tile assembly in the context of a DNA nanotube. To do this, we monitored the fluorescence of a dye molecule located at the base of a sticky end, which increases upon hybridization of the sticky end. This provides a versatile and sensitive means for monitoring assembly as a function of temperature, time and tile concentration. Results indicate the presence of nucleation barrier to assembly and a critical nucleus of >4 tiles.
*present address: Unilever R&D, UK
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