Tuesday, 24 May 2005
707

This presentation is part of: Organic Posters

Improved Method for Synthesis of DNA and RNA Containing a Thioalkyl Tether in the Minor Groove at Guanine for Crosslinking to Protein

Xiaorong Hou, Gang Wang, Barbara L. Gaffney, and Roger A. Jones. Rutgers University, Piscataway, NJ

A guanosine analog containing a thioalkyl tether with the ability to crosslink can be used to constrain the folding of DNA/RNA-protein complexes and to facilitate their structural analysis and study. We have developed a new method in which guanosine is first derivatized with a thioalkyl tether protected as the tert-butyl disulfide, and then converted into a phosphoramidite for incorporation into either DNA or RNA. After synthesis, the thioalkyl tether can be reduced to the free thiol by DTT or converted to other disulfides of varying reactivity.

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