Monday, 23 May 2005 - 4:20 PM

This presentation is part of: Spectroscopy of Biomolecules, Inferfaces and Materials II

Conformational Dynamics in Triplet Repeat Models of DNA

Benjamin J. Lee, Maryan Barch, Jens Völker, Edward W. Castner Jr., and Kenneth J. Breslauer. Rutgers, The State University of New Jersey, Piscataway, NJ

Triplet repeat diseases contain repeated sequences of (CAG) and (CTG) above a critical threshold number. Using the adenine analogue, 2-aminopurine, we investigate triplet repeat diseases by studying the temperature dependent dynamics of these triplet repeat units in hairpin, duplex, and complimentary hairpin structures using ultrafast time-resolved fluorescence spectroscopy. 2-aminopurine is used because it minimally perturbs DNA structure and also absorbs at longer wavelengths than natural DNA bases. This allows direct probing of the inner loop environment of the DNA at 3 separate sites. We are correlating changes in dynamics with a change in base stacking resulting from conformational changes in DNA.

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