Immunosensing system for Stapylococcus aureus enterotoxin B, based on electrochemical impedance spectroscopy (EIS), was developed. To achieve the sensitivity, the nano-porous aluminum substrate was electrochemically fabricated. Then, anti-S. aureus enterotoxin B was immobilized on the nano-porous aluminum substrate through a specific method, which was developed for the stability of antibody on immunosensor. Morphological changes by the binding of enterotoxin B on immunosensor was studied using atomic force microscope (AFM) and the attachment resulted in a morphological change. Especially, Z value was increased and the difference was 2nm. Even though there was only 2nm increase in Z value as enterotoxin B attachment, there was significant changes in cyclic voltammogram as well as EIS analysis of immunosensor in enterotoxin B solution showed a huge difference in resistance ranging from 20 kohm to 100 kohm at 0.25Hz. As the biological reaction between anti-S. aureus enterotoxin B and enterotoxin B, resistance decreased as time function and enterotoxin B concentration. The presence of enterotoxin could be detected in 10 minutes and linear relationship between enterotoxin B concentration and resistance could be seen after 60 minutes. This study exhibits that immunosensing system for S. aureus enterotoxin B based on EIS is sensitive and fast.
Web Page: foodsci.rutgers.edu/takhistov/Research/R1/BioChip01.htm
Back to NanoScience and Technology Posters
Back to The 37th Middle Atlantic Regional Meeting (May 22-25, 2005)