Tuesday, 24 May 2005
521

This presentation is part of: NanoScience and Technology Posters

Electroanalytical study of anti-S. aureus enterotoxin B and enterotoxin B reaction on nano-patterned aluminum surface

Changhoon Chai and Paul Takhistov. Rutgers, The State University of New Jersey, New Brunswick, NJ

Immunosensing system for Stapylococcus aureus enterotoxin B, based on electrochemical impedance spectroscopy (EIS), was developed. To achieve the sensitivity, the nano-porous aluminum substrate was electrochemically fabricated. Then, anti-S. aureus enterotoxin B was immobilized on the nano-porous aluminum substrate through a specific method, which was developed for the stability of antibody on immunosensor. Morphological changes by the binding of enterotoxin B on immunosensor was studied using atomic force microscope (AFM) and the attachment resulted in a morphological change. Especially, Z value was increased and the difference was 2nm. Even though there was only 2nm increase in Z value as enterotoxin B attachment, there was significant changes in cyclic voltammogram as well as EIS analysis of immunosensor in enterotoxin B solution showed a huge difference in resistance ranging from 20 kohm to 100 kohm at 0.25Hz. As the biological reaction between anti-S. aureus enterotoxin B and enterotoxin B, resistance decreased as time function and enterotoxin B concentration. The presence of enterotoxin could be detected in 10 minutes and linear relationship between enterotoxin B concentration and resistance could be seen after 60 minutes. This study exhibits that immunosensing system for S. aureus enterotoxin B based on EIS is sensitive and fast.

Web Page: foodsci.rutgers.edu/takhistov/Research/R1/BioChip01.htm

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