Tuesday, 24 May 2005
708

This presentation is part of: Organic Posters

Major Differences in RNA Metal Biding Motifs Determined by 15N NMR

Yupeng Fan, Barbara L. Gaffney, and Roger A. Jones. Rutgers, the State University of New Jersey, Piscataway, NJ

Divalent metal ions play both structural and functional roles in RNA. A RNA hairpin from the group I intron P5b stem loop was synthesized with [8-13C-7-15N]-guanosine at a guanine in the tandem GG·UU pair (a known metal binding site) and [7-15N]-guanosine outside the binding site for comparison. Its interactions with different metal ions in solution (Zn2+, Cd2+, Mg2+, and Co(NH3)63+) were monitored using 15N NMR. We observed very different metal binding effects compared to those we saw at a tandem GA·AG motif from the hammerhead ribozyme. (Wang, G.; Gaffney, B. L.; Jones, R. A. J. Am. Chem. Soc. 2004 126, 8908 -8909)

 


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