Monday, 23 May 2005 - 11:45 AM
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This presentation is part of: Nanoparticles, Microparticles and Vesicles

Synthesis and Characterization of Collagen Mimetic Peptide Conjugated Gold Nanoparticles

Xiao Mo, Yoojin An, Allen Y. Wang, and Michael S. Yu. The Johns Hopkins University, Baltimore, MD

While nanoparticle conjugates have been used in the past as immuno markers and cellular uptake tracers, applications in a broader biomedical discipline are greatly limited by their instability in aqueous conditions. Here we present a “bio-mimic” approach for generating highly stable, water-soluble gold nanoparticles that are passivated with collagen mimetic peptides (CMPs). CMPs are non-ionic peptides composed of Pro-Hyp-Gly trimer repeats, a representative peptide sequence of natural collagen molecules. The CMP-Au nanoparticle conjugates are stable in a wide range of pH and can endure saturated sodium chloride solution without aggregating. The CMP-Au can be freeze-dried and subsequently re-dissolved to yield a stable dispersion. UV-vis spectroscopy, dynamic light scattering, and transmission electron microscopy were used to characterize the CMP-Au nanoparticle conjugates. Stability of nanoparticles was evaluated with respect to the peptide length and sequence. The CMP-Au nanoparticle conjugates can be readily derivatized with other biomolecules using the carboxyl groups at the C-termini of the peptide chains. Due to the superb stability, the CMP-Au nanoparticle conjugates can be used in applications that may involve relatively harsh solvent condition such as high ionic strength, or for situations that may involve wide range of solvent polarity.

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