Sunday, 22 May 2005

This presentation is part of: Pre-College Reseach Posters

Can Cruciferous Vegetables Repair Damaged Cell Cycle Checkpoints?

William J. Zupko, Woodbridge High School, Fords, NJ

Indole - 3 - Carbinol is a commonly found chemical in cruciferous vegetables such as broccoli. Many reports suggest that these vegetables provide cancer protection by correcting damaged cell cycles. I was interestied in finding out if the Indole 3 Carbinol does play a role in the repair of the damged cells. I experimented with the Indole - 3 Carbinol, and tested it with mutant yeast (S pombe), which don't divide at 35 degrees Celsius, to see if the yeast would once again reproduce and divide. During my experimentations, I had found out that Indole - 3 - Carbinol is not soluble in water slightly in alcohol, and more in acetone. When I applied the Indole - 3 - Carbinol to the yeast while suspended in acetone, the yeast were killed by the concentration of acetone. After I exhausted all possible ways of applying the Indole - 3 - Carbinol to the yeast, I used a broccoli extract suspended in alcohol. First I mixied the broccoli extract into the agar plate, yet none of the yeast grew. I also tried applying 0.5ml of broccoli extract on top of the yeast and had again received no results from the yeast that were placed in 35 degrees however, those yeast that were placed at room temperature grew. These yeast cells had taken a longer amount of time to grow, and were not all rod shaped as the mutant yeast should be, but appeared shorter, and slightly rounded.

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