A third generation triazine-based dendrimer containing 6 chlorotriazines and 12 hydroxyl groups on the surface was synthesized using the divergent approach. Ease of synthesis and orthogonal functionality on the exterior of the parent hexa-chlorotriazine dendrimer offers an efficient route to derivatize a series of dendrimers, each being monodisperse and fully characterizable. The parent dendrimer was synthesized in seven steps with a 60% overall yield, and further manipulation of the hexa-chlorotriazine dendrimer was achieved through nucleophilic aromatic substitution with a series of bifunctional amines. This library of dendrimers is of interest for transfection assays due to their cell trafficking ability while interacting with siRNA as well as other transfection agents. Using the parent dendrimer as a platform for further functionalization offers reactive sites to attenuate cytotoxicity. Upon completion of the biological studies on the whole library of dendrimer architectures, variations in functionality will be used to understand the relationship between functional groups, transfection ability, and toxicity. Preliminary transfections studies and results are expected for inclusion.