This research details the first use of hydrophobically modified chitosan to modify electrode surfaces. Chitosan was modified by reacting a saturated alkyl aldehyde with the free amino group on the chitosan backbone. The modified chitosan was resuspended in a 1% by weight mixture in t-amyl alcohol, chloroform, or 50% aqueous acetic acid. It was shown that hydrophobically modified micellar polymers alter the transport properties to the electrode surface as a function of size and charge of the redox species. Fluorescence microscopic assays and cyclic voltammetry were used to determine the optimal modified polymer for effective enzyme immobilization. Enzyme immobilization was carried out with alcohol dehydrogenase, aldehyde dehydrogenase, formaldehyde dehydrogenase, formate dehydrogenase, lactic dehydrogenase, and glucose dehydrogenase. Future work involves modifying chitosan with the redox mediator Ru(bpy)₃²⁺, which also functions as the hydrophobic modifier, allowing the chitosan to form the micelle structure necessary for enzyme immobilization.