Panax ginseng is an herbal medicine used in Chinese medicine and is being reported to posses various biological activities such as protein anabolic and antidiabetes effects, antitumor activities, and prevention of the aging process. The active groups in panax ginsengs are O-glycosides of dammarene-type triterpene saponins known as ginsenosides. More than 20 of these ginsenosides have been isolated and characterized. The most important powerful and reliable analytical technique for studying these compounds is liquid chromatography combined with mass spectrometry (LC/MS) in its both positive and negative ion modes. The main objective behind this research is to study the effects of a combination of ginsenosides on cancer. This will be performed by treating cancerous mice with a combination of four different ginsenosides. The concentrations of these ginsenosides and their metabolites in the blood will then be monitored using LC/MS and correlated with the physiological effects on the mice. Data about LC/MS optimization to obtain high sensitivity and optimization of ginsenoside extraction methods from serum samples will be presented.