Abstract: Poly-L-lysine-Myoglobin films attached to pyrolytic graphite covalently are relatively stable, even at 2000 rotations per minute for 12 hours in both microemulsions (΅Es) and buffer solution. Remarkable Mb protection at pH 2.0 was observed in both SDS and CTAB microemulsions, unlike in aqueous buffer as shown from both circular and visible spectroscopy. Redox formal potentials in these films were most positive at pH 2.0 and most negative at pH 12.0 in all the media studied. These films supported the reduction of tertbutylhydroperoxide, hence the production of ferrylmyoglobin (X-MbFeIV). Results on these films were consistent with Michaelis-Menten and Koutecky-levich models. In both SDS and CTAB microemulsions, the turnover rates, kcat, were highest at pH 2.0 and 12.0 than at neutral pH. But in buffer solution, the order was: pH 2.0 > 6.5 > 12.0. Changes in microemulsions composition had less influence on Michaels's dissociation constant (Km) and myoglobin efficiency and specificity (kcat/Km).
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