Near-field scanning optical microscopy (NSOM) provides optical resolution at the nanometer scale, so optical studies can now be done beyond the diffraction limits of classical optics. Here spatially resolved near-field transmission images of annealed gold island films as well as near-field fluorescence images of the dye labeled phospholipid monolayer deposited on the top of the films by the Langmuir Blodgett technique were studied. The location of the lipid molecules can be determined to ~50nm with analysis of the near-field fluorescence signal from the labeled lipids. Simultaneously, the near-field transmission signal from the metal island film can map the position of the nano-clusters and crevices on the metal surface. Overlaying of the near-field transmission and fluorescence images from the same area of the sample shows local transmission of gold island film is at a minimum where the fluorescence of the lipid monolayer is at a maximum. It was concluded that coverage of the metal island film by the Langmuir-Blodgett phospholipid monolayer is incomplete, and lipid molecules are preferentially localized in crevices of the film.