Electrospun fabrics of differing hydrophilicities were used as substrates for biosensor assemblies. Electrospun fibers have very small diameters which allow the fabrics they compose to have ultra-high surface areas. This factor makes it possible to affix a large quantity of biorecognition elements to the membranes. To make these fabrics, solutions of cellulose acetate were prepared and electrospun for three hours. The fabrics were then subjected to either no deacetylation (remaining cellulose acetate-low hydrophilicity), complete deacetylation to form cellulose (high hydrophilicity), or partial deacetylation to form an intermediate of cellulose acetate and cellulose (intermediate hydrophilicity). FTIR analysis confirmed that the desired deacetylations were achieved. Contact angle tests and wicking/liquid migration studies determined the relative hydrophilicities were as expected. Porosity tests have shown that the deacetylation process caused a reduction in pore size, which has implications for the migration of the biosensing elements up the electrospun strips. Tests involving the use of these electrospun fabrics as membranes for biosensor assemblies which make use of biotin-streptavidin specific binding are currently being undertaken. Results will allow for a comparison of effectiveness based on membrane hydrophilicity.