NanoLC with 75µm id columns and flow rates of 200nL/min is gaining in popularity due to improved resolution, lower sample injection requirements, and better ionization efficiency leading to improved sensitivity. NanoLC peaks typically elute within 20s, providing most modern mass spectrometers sufficient time to perform MS/MS for simple protein ID experiments. However, for complex samples, such as glycopeptides where MS3, MS4, and MS5 experiments may be needed, nanoLC does not provide adequate analysis time. By collecting fractions from the nanoLC column, analysis times can be extended via lower flow nanospray infusion analysis. Additionally typically for LC/MS experiments, one must sacrifice ionization efficiency for optimal chromatography. It would be desirable to decouple chromatography from ionization, allowing the two events to be optimized independently from one another. Here, we demonstrate a novel Nano Fraction Analysis Chip Technology (nanoFACT) capable of automated ultra-low fraction volumes from 75µm nanoLC to 300µm ID capLC columns followed by subsequent automated nanoelectropray analysis for increased data content via mass spectrometry. Fractions from capillary columns were collected every 60-90s sec from a column flowing at 200nL/min with a 30m gradient. Fractions were collected from the peak elution window of interest in an automated fashion using a robotic nanoelectrospray system (TriVersa NanoMate). Following fraction collection and sample dry down the residue in each tip was reconstituted in ~200 nL. The sample was analyzed directly from the pipette tip with 2.5µm ID chip-based nanoelectrospray emitters operating at ~29-40 nL/min. Test samples include RNaseB, fetuin, and a yeast crude cell extract.