We investigated methods for covalently grafting microtubules onto functionalized glass surfaces based on the surface reactions of primary amines. Previous work has shown that coating glass surfaces with 3-aminoproplyethoxysilane (APTES) immobilizes microtubules on glass surfaces using electrostatic interactions. Our goals are to covalently immobilize microtubules in order to 1) bind microtubules more strongly on glass surfaces 2) exploit more precise surface chemistries to enhance the results from atomic force microscopy (AFM) studies of microtubules. Glass surfaces were initially amino-silanized using APTES, followed by the attachment of succinimidyl ester groups using the homobifunctional cross-linker, di(N-succinimidyl) carbonate, and lastly, the immobilization of the microtubules on the surface through the remaining N-hydroxysuccinimide (NHS) linker. Our results show that the attachment of homobifunctional cross-linker to the glass surfaces increases the immobilization of microtubules from optical microscopy studies. We also investigated ways to completely passivate glass surfaces using polyethylene glycol (PEG). N-hydroxysuccinimide ester PEG molecules containing spacer arm lengths of 4 and 12 subunits in two separate treatments were attached to APTES-functionalized glass surfaces. We determined that passivation of the glass surfaces worked best using Methyl-PEO₁₂-NHS Ester, which had a longer PEG spacer arm, under alkaline conditions. While PEGylation of glass surfaces greatly reduces protein adsorption, we are currently investigating the use of surface-bound dendrimers to increase the amine concentration on the glass surfaces in order to enhancee passivation and immobilization using the above approaches. The results of these studies should improve our ability to control microtubule adsorption on surfaces surfaces for AFM studies.