The development of practical biosensors for the detection and quantification of contaminants in environmental samples is a subject of considerable research. A new biosensor platform based on polyamic acid (PAA); a functionalized conducting polymer substrate that provides electrochemical detection and control of biospecific binding has been developed and investigated using antibody-antigen interactions. Specifically, Goat anti-mouse was covalently bound to a PAA modified gold working electrode. Water soluble carbodiimide, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysulfosuccinimide (NHS) were used to activate and cross-link the carboxyl end groups of the PAA to Goat anti-mouse. The chronoamperometric responses of the biosensor showed a linear variation with different concentrations of the mouse IgG using the PAA-antibody modified biosensor platform. This presentation will focus on the synthesis and the use of surface, structural and morphological characterization techniques for PAA-antibody modified gold electrode in biosensing.