The fabrication of nanostructured materials through self-assembly of nanoscale building blocks has attracted global interests in recent years because of their wide range of applications such as optical coatings, microelectronics, information storage, bioanalytical probes, and chemical/biological sensors. One area of challenges is the ability to control the assembly of nanoparticles at molecular or nanoscale scale. In this poster, the results of an investigation of the growth of the nanoparticle film assemblies from different nanoparticle sizes and molecular linkers or mediators will be described. The surface plasmon resonance optical properties are studied to assess the thin film growth as functions of different control parameters. The chain length of the linker molecules is found to not only affect on rate of thin film growth, but also on the optical properties of thin film. The correlation between the chain length and the optical properties will be discussed. The results provide new insight into the fine-tuning of the interparticle spatial property and its role in the electronic and optical properties of the thin film assemblies.