Zhao Deng1, Tiffany Zink1, Huan-Yuan Chen2, Fu-Tong Liu2, and Gang-yu Liu1. (1) University of California, Davis, Davis, CA, (2) University of California, Davis, Sacramento, CA
A combined confocal laser scanning microscopy (CLSM) and atomic force microscopy (AFM) is utilized to investigate the morphological changes of mast cells upon IgE cross-linking by dinitrophenyl-bovine serum albumin (DNP-BSA). The high-resolution AFM topography acquired in PBS reveal the 3D morphological changes of the mast cell membrane upon activation. Membrane ridges with height ranging from 1 to 2 ėm are characteristic features for activated cells. Rich structural information of these mast cells at various stages of growth and activation will also be presented.
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Back to The 61st Northwest Regional Meeting (June 25 - 28, 2006)