Monday, June 18, 2007 - 9:10 AM The Kestrels (Boise Centre on the Grove) 74
Novel Waveguide Structures for Bioassays with Broad Applications to Analytical Instrumentation
Heather Edberg, Richard Ozanich, R. Shane Addleman, Michael Wojcik, Summer Ziegler, and Kathryn Antolick. Pacific Northwest National Laboratory, Richland, WA
Many current fluorescent bioassay systems employ optical fibers for excitation/illumination of the fluorescent reporter and fiber collection of the emitted fluorescence, limiting the number of photons that can be collected. By collecting a larger portion of the fluorescent signal, the detection limits of the on-column bioassay can be reduced. Conversion of the bioassay flow cell to a liquid core waveguide for sensitive on-column detection of fluorescence-based bioassay signals will be covered. The current flow cell is constructed from FEP Teflon with a refractive index of 1.35. For waveguide conditions to exist, the refractive index of the flow cell contents must be greater than the flow cell. This presentation explores the modification of the refractive index of the flow cell through fabrication of the flow cell from a lower refractive index material.