Monday, June 18, 2007 - 1:30 PM The Kestrels (Boise Centre on the Grove) 158
Detection of Interleukin-6 Based on Dye-Doped Silica Nanoparticle Label/Protein Microarray
Hong Wu, Qisheng Huo, Guodong Liu, Jun Wang, Susan Varnum, Zimin Nie, Jun Liu, and Yuehe Lin. Pacific Northwest National Laboratory, Richland, WA
The fluorescent labelling has been widely used in biological analysis. We report a dye encapsulated silica nanoparticle with the advantages of high fluorescence intensity, photostability and biocompatibility as label for protein microarray fluorescence immunoassay. The Rubpy dye labeled nanoparticle was characterized by transmission electron microscopy (TEM) and fluorescence spectroscopy. It was found that the sizes of the nanoparticles are uniform with diameter of 50 nm and there are approximately 9,000 dyes per silica nanoparticle based on calculation. The highly bright nanoparticles improve the detection sensitivity with the limit of detection of biomarker IL-6 down to 0.1 ng/mL. The calibration curve ranges from 0.1 ng/mL to 10 ng/mL. The capability of detection IL-6 in spiked human plasma sample provides the great opportunity for clinical diagnosis and prognosis of IL-6 related diseases.