Flavonoids are one of the most important classes of plant metabolites. Compounds from this class are involved in controlling important aspects of plant growth, development, reproduction, and interactions with the environment. The ability to produce flavonoids was requisite for plants' adaptation to a terrestrial environment and represents one of the earliest adaptations of plants to a landscape bathed in UV light. Flavones, a subclass of the flavonoids, play this and other roles in the plant. Flavones are also very important for human health. In addition to anti-bacterial, anti-viral and anti-fungal properties, which they possess, many flavones are very important antioxidants and now appear to play roles in improving cognition and protecting the brain from damage associated with aging or Alzheimer's disease. Sweet basil and peppermint are members of the mint family of plants and are well known to possess high levels of a diversity of flavones. These compounds are produced in these plants in specialized structures on the surface of the leaf known as peltate glandular trichomes. In order to elucidate the biosynthetic pathway(s) to these important compounds in these plants, we must have a firm understanding of the levels of different flavones in the plants at different developmental stages and in different basil lines. In this investigation, we use a metabolic profiling approach that utilizes tandem mass spectrometry coupled to HPLC to identify and quantify the flavones in these plants. Differences in flavone profiles between different basil lines, between basil and peppermint, and through plant development will be discussed.