The synthesis of goat anti-rabbit and goat anti-mouse horseradish peroxidase (HRP) conjugates has been performed using a reproducible, straight-forward method, and exhibit both optimum performance and enhanced stability. The two components were combined to give a relatively small, well-defined conjugate of approximately 300 kDa. A cocktail of the goat anti-rabbit and goat anti-mouse HRP conjugates were tested across a panel of twelve primary mouse and rabbit antibodies alongside the second generation Nichirei HRP polymeric conjugate. In all cases, the HRP-IgG conjugate equaled or outperformed the competitive staining system. Thermal stability of the goat anti-mouse and goat anti-rabbit conjugates was evaluated against the Nichirei HRP polymer. Comparative staining data illustrated the robustness of the conjugate over the Nichirei system after storage for one week at 45oC.