We have developed a novel method of linking haptens to cytidine via thermal bisulfite catalyzed transamination with hydrazines. DNP hapten was tethered to dCTP via a non-immunogenic PEG spacer utilizing a novel hydrazine linkage. The synthetic route followed offers a new alternative to the current method of linking haptens to cytidine. This synthetic approach employs microwave catalyzed hydrazinolysis that enables the attachment of spacers via hydrazine linkages. The resulting nucleotide triphosphate can serve as a unique substrate for the enzyme mediated labeling of DNA probes. The efficacy of incorporation of the new linker was tested in the nick translation labeling reaction of an HPV probe cocktail. The labeled probe was subsequently tested in an ISH assay on mouse xenograft model system. We were able to detect signal in CaSki (400-600 copies of HPV 16), HeLa (10-50 copies of HPV 18), and SiHa (1-2 copies of HPV 16) xenograft tissue slides and CINIII/Cancer patient samples. The signal was strong and easy to interpret.