The recent increase of the threat of bioterrorism has made the detection of potentially harmful biological agents an extremely important topic. Detection strategies are needed which utilize non-toxic, cost-effective simulants capable of mimicking the antigenicity of these agents. One such biological agent is the hemorrhagic Ebola virus. We have designed a nanoscale complex that mimics the antigenicity, and thus the immunogenicity, of the Ebola glycoprotein. Epitope mapping produced a 17 a.a. epitope of the glycoprotein, which when covalently attached to a support scaffold, such as Au monolayer protected clusters, is able to recapitulate the relevant structure of the glycoprotein. The ability of this peptide-nanocluster to recognize and bind a monoclonal antibody specific for the native glycoprotein was then shown via ELISA and QCM. The degree of binding of this mimic, as measured via equilibrium and kinetic constants, compares favorably with values shown for the native glycoprotein.