Giso Abadi1, Thomas Potter2, Thomas J. Manning3, Paul Groundwater1, Lyn Noble1, James Nienow3, Dennis Phillips4, Russ Goddard3, Jerry Purcell5, Alan G. Marshall5, and Carol Nilson5. (1) Sunderland University, Sunderland, United Kingdom, (2) USDA, Tifton, GA, (3) Valdosta State University, Valdosta, GA, (4) University of Georgia, Athens, GA, (5) Ion Cyclotron Resonance Program, Tallahassee, FL
Bryostatin is a marine natural product that is produced by marine bacteria that is symbiotic with the bryozoa Bugula neritina as well as a host of other marine organisms in the same ecosystem. In this study we use a scanning electron microscope (SEM) to study several artificial mediums manufacturer in this lab that host marine bacteria and from which bryostatin has been extracted. While it is difficult or impossible to distinguish bacteria species visually, it can be used to quantitate the overall bacterial density in mediums that are producing bryostatins. Bryostatins are quantitatively and qualitatively identified with Liquid Chromatography-Mass Spectrometry (LC-MS), Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometer (MALDI-TOF-MS) and Fourier transform-Ion Cyclotron Resonance (FT-ICR). Many of our artificial mediums contain micrometer sized materials so separating and utilizing typically counting methods provide inaccurate measurements.