With the earth's ozone layer depleting, studying and understanding organisms responses to short wavelength Ultra-Violet light has become a growing area of research. In plants, the UV response involves a signaling pathway that calls for changes that can protect the plant from damage. It is thought that most of the change occurs due to an increase in production of polyphenolic compounds called flavonoids that are responsible for much of the plants pigmentation. The flavonoids act as the plants “sunscreen” and will protect the plant from further damage sustained from the UV exposure. Capillary Electrophoresis (CE) was used to characterize increases in flavonoid production upon exposure to UV light and a Photodiode Array Detector (PDA) was used for its capability to take the UV-Spectrum of specific peaks. Samples were extracted from Cucumber and Arabidopsis plants. Both plants were harvested in embryonic stages of development and pigments were extracted with 100% Methanol. Initial work with standard UV-Spectroscopy revealed that pigment absorbance increases at 265 and 330nm. CE runs with cucumber extracts showed differences that correspond with original results from UV-Spectrum. Work with Arabidopsis extract is currently moving forward, especially in optimizing extraction procedures.