The Mediterranean gecko (Hemidactylus turcicus) is an invasive, ubiquitous species in the Southern United States that is found in a broad spectrum of diverse habitats. Because these reptiles often inhabit industrialized areas, we analyzed the concentrations of eight metals from the tissues of Mediterranean geckos collected in Calcasieu Parish (which is heavily industrialized), and in Beauregard Parish (which is rural). Geckos were caught by hand, measured and weighed to estimate body condition, and then a section of each lizard's tail was collected for analysis. Gecko tail sections were dried, and then analyzed for Pb, Cu, Zn, Cd, Cr, Fe, Mn, and Ni content using an inductively coupled plasma instrument. There were no statistically significant differences detected for any of the eight metals in the samples collected from Calcasieu and Beauregard Parishes. There were also no significant correlations between metals concentrations and gecko body condition. However, females from a site in Westlake had significantly greater concentrations of Zn, Fe, and Cu than did females from a site in Moss Bluff about 10 km away (both in Calcasieu Parish); there were no significant differences between males from these two sites. Overall, females at each site had higher concentrations of all eight metals than males from the site (except for Zn at Moss Bluff), with the differences reaching the level of statistical significance (p<0.05) for Cu, Cd, Cr, and Ni at the Westlake site. This suggests there are sex-based differences in metal accumulation in this species.