123 Genetic STR Typing Using Microflometric Technology

Wednesday, November 4, 2009: 9:20 AM
Longhorn (Camino Real Hotel)
Caitlin Lott , Department of Chemistry, St. Edwards University, Austin, TX
Casie Parish, M.S. , Department of Criminal Justice, Criminology and Forensic Science, St. Edwards University, Austin
Donald Wharry, Ph.D. , Department of Chemistry, St. Edwards University, Austin, TX
Eamonn Healy , Department of Chemistry, St. Edward's University, Austin, TX
The purpose of this research is to use Real Time PCR and the Agilent Bioanalyzer as a low cost substituted method for the Genetic Analyzer in which we produce discriminating genetic profiles for known and unknown subjects. The main focus of the project is to utilize the core 13 CODIS loci which exhibit tetra repeat sequences. Currently, TPOX is being tested, and future research will expand to other CODIS loci. The samples include known DNA samples as well as buccal swab DNA that has been extracted using micro-bead technology. Both homozygous and heterozygous peaks are studied to broaden the scope of the study as well as utilizing different degrees of repeat separation. Profiles produced will then be compared to determine a statistical value for reproducibility and accuracy of results.
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