29 Method Development for the Analysis of DDT, DDE, DDD in Cow Milk

Wednesday, November 4, 2009
Ballroom A+B (Camino Real Hotel)
Amanda M. Parra , Chemistry, University of Texas at El Paso, El Paso, TX
Beatriz Rocha , Chemistry, University of Texas at El Paso, El Paso, TX
Wen-Yee Lee , Department of Chemistry, University of Texas at El Paso, El Paso, TX
Organochlorine Pesticides (OCs) are classified as persistent organic pollutants (POPs). OCs may be responsible for a vast number of health conditions including neurological disorders, endocrine disruption, reproductive dysfunctions, and possible linkage to cancer. Due to their toxicity in human and wildlife, OCs such as dichlorodiphenyltrichloroethane (DDT) and its metabolites dichlorodiphenyldichloroethylene (DDE) and dichlorodiphenyldichloroethane (DDD) are banned. However, they still persist in the environment and have been detected in environmental samples worldwide. These compounds tend to attach to fatty tissues in animals and humans. DDT and its metabolites (DDTs) have been detected in human adipose tissues and in breast milk. Research has been conducted worldwide to detect DDTs in breast milk for assessing the health impact of DDTs on infants.The main objective of this study is to develop a new extraction method to determine the presence of DDT, DDE and DDD in milk using an innovated and environmental friendly technique, called Stir Bar Sorptive Extraction (SBSE). The polymer coating on the stir bar attracts organic compounds and can extract DDTs from the solution. SBSE coupled with thermal desorption Gas Chromatography and Mass Spectroscopy will be applied in the analysis of OCs in samples. Different solvent systems and additive effects were tested with SBSE process to determine OC recovery in milk samples. It was discovered that a 30% mixture of acetonitrile/ water and sodium hydroxide as an additive yielded the most OC recovery after extraction.
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