97 Curcumin as a Hydroxyl Radical Scavenger

Wednesday, November 4, 2009: 4:40 PM
Kohlberg (Camino Real Hotel)
Robert Viņa-Marrufo , Department of Chemistry, University of Texas at El Paso, El Paso, TX
Amber S. Harris , Department of Chemistry, University of Texas at El Paso, El Paso, TX
Amalia Vasquez , Department of Chemistry, University of Texas at El Paso, El Paso, TX
James E. Becvar , Department of Chemistry, University of Texas at El Paso, El Paso, TX
Mahesh Narayan , Department of Chemistry, University of Texas at El Paso, El Paso, TX
The spice component curcumin, from turmeric, is believed to be a scavenger of reactive oxygen species. Nitric oxide (NO.) and hydroxyl (OH.) radicals are known to be causative agents in several diseases. The ability of curcumin to scavenge: 1) hydroxyl radical produced through Fenton's reaction and 2) nitric oxide radical formation with nitric acid was investigated. Curcumin's effect on the reactive oxygen species was studied using lumitection analysis (US Patent 6017722) involving inhibition of Vibrio harveyi, Vibrio fischeri, and Photobacterium leiognathi bioluminescence. Mass spectrometry indicated that curcumin may be scavenging both nitric oxide and hydroxyl radicals. Lumitection analysis showed an increased acute and chronic inhibition of bioluminescence in these common strains of bioluminescent bacteria.