34 Determination of Estrogenic Activity of Endocrine Disrupting Compounds in Wastewater Using Chemiluminescent Yeast Estrogen Screening

Wednesday, November 4, 2009
Ballroom A+B (Camino Real Hotel)
Ricardo McCreary Jr. , Department of Chemistry, University of Texas at El Paso, El Paso, TX
Roberto De La Torre-Roche , Department of Environmental Science and Engineering, University of Texas at El Paso, El Paso, TX
Marc B. Cox , Department of Biology, University of Texas at El Paso, El Paso, TX
Wen-Yee Lee , Department of Chemistry, University of Texas at El Paso, El Paso, TX
Several endocrine disrupting compounds (EDCs)-estrone (E1), estradiol (E2), nonyphenols (NP), and bisphenol A (BPA)-are commonly present in the influent and effluent from wastewater treatment plants.  Studies have shown that these compounds can have adverse effects (e.g. reproduction and developmental effects) on aquatic organisms, even at very low concentrations (ng/L), with serious implications for affects on human health. Due to their impact on the environment and their occurrence at very low concentrations in wastewater and surface water, sensitive chemical and biological procedures are needed to determine the estrogenicity of these compounds in water samples.  In this study,Gas chromatography-Mass spectrometry was used for the chemical analysis of EDC concentrations and chemiluminescent yeast estrogen screening (CYES) was used to determine estrogenic activities.  Three different treating methods were performed (CYES) to compare their sensitivity in measuring the total estrogenic activity in influent and effluent wastewater samples.  In one method yeast cells were treated directly with the wastewater samples. The other methods involved the preliminary extraction of the EDCs from the wastewater samples via solid phase extraction (SPE)-differing in that one method was ran through Silica Gel--before treatment of the cells. Simultaneously, water and extract from from the extraction procedures were analyzed to determine EDC concentrations. Results for the chemical analysis and the CYES, represented as ng/L of Estradiol Equivalents (EEQs), will be reported.  Comparing the biological and chemical analysis will allow us to determine the dominant contributors of estrogenic activity in the wastewater.
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