248 ROLE of Southwestern ENVIRONMENTAL Airborne Particulate MATTER (PM)-ASSOCIATED Polycyclic Aromatic Hydrocarbons (PAHS) IN HUMAN Lung Epithelial CELL Inflammatory RESPONSES

Thursday, November 5, 2009: 11:00 AM
Kohlberg (Camino Real Hotel)
Scott W. Burchiel , Center for Environmental Health Sciences, The University of New Mexico, Albuquerque, NM
Previous work form our lab has shown that polycyclic aromatic hydrocarbons (PAHs) are associated with PM 10 and PM 2.5 micron particles obtained from the Paso del Norte air basin. We found temporal and spatial variation in the amounts and types of PAHs associated with PM, with highest levels detected in winter months. Organic extracts of PM 10 and PM 2.5 filters demonstrated that several sets of genes were induced in the human bronchial epithelial cell line BEAS2B following in vitro exposures. These included: 1) inflammation-associated genes, 2) oxidative stress-associated genes, and 3) aromatic hydrocarbon receptor (AhR)-associated genes. Using RT-PCR we compared the activities of PM extracts obtained from different regions and seasons in El Paso. We also examined whole human genome gene arrays and identified additional potential target genes in BEAS2B cells. The results of these studies demonstrated that PAH mixtures are important in inducing disease-associated target genes in human lung epithelial cells. There are also likely important interactions between PAHs and other agents, such as metals, that may contribute to lung diseases. Therefore, current studies are assessing the interactions between PAHs and other airborne toxics that may contribute to environmental pollution and human lung diseases.
Abstract based upon: Lauer, FT, Mitchell, LA, Bedrick, E, McDonald, JD, Lee, W-Y, Li, W-W, Olvera, H, Amaya, MA, Berwick, M, Gonzales, M, Currey, R, Pingitore, NE Jr, and Burchiel, SW. (2009) Temporal-Spatial Analysis of U.S.-Mexico Border Environmental Fine and Coarse PM Air Sample Extract Activity  in Human Bronchial Epithelial Cells, Toxicol Appl Pharmacol,  238, 1-10.
This work was supported in part by NIEHS S11 ES013339-01A1 at the University of Texas at El Paso (Pingitore, PI) and the New Mexico Center for Environmental Health Sciences NIEHS P30  ES012072 (Burchiel, PI).