306 Dye-Sensitized Ruthenium Nitrosyls: Trackable NO Donors for Light-Triggered NO Delivery to Cellular Targets

Thursday, November 5, 2009: 4:20 PM
Ballroom C+D (Camino Real Hotel)
Pradip K. Mascharak, Professor of Chemistry and Biochemistry , Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA
The discovery of the diverse roles of nitric oxide (NO) in biology has raised high level of interest in the design and syntheses of exogenous NO donors to modulate physiological pathways such as blood pressure regulation, platelet adhesion, and immune response. Since higher levels of NO also cause cellular apoptosis, NO donors have been used to kill tumor cells of different origin and grade. In order to deliver high levels (micromolar and above) of NO to selected targets under controlled conditions, we have designed photoactive metal nitrosyls that can be triggered by lights of specific wavelengths.  The design also allows one to track such NO donors in cellular environment via smart use of dye molecules attached to the nitrosyls. Two such designed nitrosyls have been employed to cause NO-induced apoptosis in human breast cancer cells under the total control of light.  The design principles and their utility as trackable NO donors in cellular (aqueous) environment will be discussed.