Tuesday, 24 May 2005 - 9:50 AM
479

This presentation is part of: Graduate Presentations

Influence of Bead Size on Activity and Distribution of Candida Antarctica Lipase B (CAL-B) Adsorbed on Macroporous Polyacrylic Beads

Bo Chen and Richard Gross. Polytechnic University, Brooklyn, NY

ABSTRACT: Adsorption of Candida Antarctica Lipase B (CAL-B) on macroporous polyacrylic beads under the trade name of AmberchromÖ with various diameters but uniform pore size and surface area has been studied. A decrease in particle size results in a increase of CAL-B adsorption as well as the increase of the specific activity in ring-opening polymerization of ň- caprolactone in deuterated toluene and polycondensation of adipic acid and 1,8- octanediol in bulk. The amount of enzyme retained activity was determined by active site titration using a organic phosphate, methyl p-nitrophenyl n-hexylphosphonate (MNPHP) as the inhibitor. Moreover, a change of protein distribution upon adsorption is observed using IR microscope by scanning the concentration of amide bond on the film which is cut from CAL-B immobilized beads. CAL-B entered the center of beads when their size decreases to 35 ým while it stayed on the shell (~40ým) of beads when their size is more than 120ým. These results indicate that the size of beads influences the concentration, distribution and the activity of adsorbed CAL-B.

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