Monday, 23 May 2005 - 2:50 PM

This presentation is part of: Gene Expression: Transcription

Complexes of CAP in Transcription Activation

Catherine Lawson, Andrew A. Napoli, Brian Benoff, Helen M. Berman, Yon W. Ebright, and Richard H. Ebright. Rutgers University, Piscataway, NJ

Global regulators control cellular behavior by turning on many genes at once in a coordinated way. The catabolite activator protein (CAP) is a global regulator of E. coli and is the most extensively studied member of a large, ubiquitous family of structurally related regulators in bacteria. Transcription activation by CAP is substantially simpler than most examples of transcription activation in bacteria and eukaryotes, which can involve complex arrangements of macromolecular components and/or DNA binding sites. Structural knowledge of CAP and RNA polymerase (RNAP) and their interactions with promoter DNA elements is quite advanced. In contrast, knowledge about about the interactions between CAP and RNAP on promoter DNA in the two simple classes of transcription activation is still based largely on biochemical and genetic data. We will present our progress towards obtaining high-resolution structures of sub-assemblies representing Class I and Class II CAP-RNAP-promoter DNA complexes.

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