Monday, 23 May 2005

This presentation is part of: Biological Chemistry Posters

The Influence of Abasic Sites on the Self-Assembly of DNA Quadruplexes

Cosimo Antonacci and Richard D. Sheardy. Seton Hall University, South Orange, NJ

The self assembly of G-rich DNA sequences into quadruplex structures has been extensively noted in the literature and has been implicated in biologically relevant segments of the genome, such as telomeres. Quadruplexes are composed of G-quartets stabilized by four coplanar guanines in a Hoogstein base pairing motif forming a quartet pore capable of housing mono and, to a lesser extent, divalent cations. We are interested in observing the G rich sequences TXG4 and DXG4, where x=1,2 and 4 and D is a nucleotide without a base. The purpose is to examine the thermodynamic contributions of the T base versus no base and to identify the effect of 5' T elongation on quadruplex stability in 100 mM potassium phosphate buffer. We will present results from Circular Dichroism, Differential Scanning Calorimetry and UV-Vis studies.

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