A guanosine analog containing a thioalkyl tether with the ability to crosslink can be used to constrain the folding of DNA/RNA-protein complexes and to facilitate their structural analysis and study. We have developed a new method in which guanosine is first derivatized with a thioalkyl tether protected as the tert-butyl disulfide, and then converted into a phosphoramidite for incorporation into either DNA or RNA. After synthesis, the thioalkyl tether can be reduced to the free thiol by DTT or converted to other disulfides of varying reactivity.
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Back to The 37th Middle Atlantic Regional Meeting (May 22-25, 2005)