Tuesday, 24 May 2005 - 3:10 PM
601

This presentation is part of: College Student Award Symposium sponsored by the Chromatography Forum of Delaware Valley

Effect of Pseudostationary Phase on Fluorescence Intensity in Electrokinetic Chromatography

Stephanie A. Schuster and Joe P. Foley. Drexel University, Philadelphia, PA

The routine use of capillary electrophoresis as a separation technique has been hampered by its lower sensitivity compared to HPLC. Several methods have been developed to overcome this problem including preconcentration techniques (stacking), enhanced path length capillaries (bubble cell, Z cell), and the use of Laser Induced Fluorescence (LIF) as a detection method. LIF also has its disadvantages, including the need to fluorescently label non-fluorescing analytes. Researchers have discovered that the incorporation of a pseudostationary phase (PSP), such as micelles or cyclodextrins, in the background electrolyte (BGE) can enhance the fluorescence intensity of the investigated compounds. This study compares the fluorescence intensity of a set of probe analytes (derivatized amino acids) in the presence and absence of two PSPs: (i) sodium dodecyl sulfate (SDS) micelles and (ii) cetyltrimethylammonium bromide/sodium octyl sulfate (CTAB/SOS) vesicles.

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