Pain is a mechanism used by multicellular organisms to avoid potentially harmful stimuli in the environment. The receptor TRPV1 is found on the cell surface of pain-sensing neurons and is activated by several different stimuli: noxious heat (>45°), low pH, and vanilloids such as capsaicin, the active ingredient in ‘hot' chili peppers. We have expressed an amino-terminal fragment of TRPV1 in Escherichia coli as a GFP-fusion protein. The expressed protein requires the addition of chaotropic agents for solubilization. We are currently optimizing methods for re-folding the protein. The structural integrity of the fragment is being characterized using several biochemical and spectroscopic methods.
Back to Biological Chemistry Posters
Back to The 37th Middle Atlantic Regional Meeting (May 22-25, 2005)