Monday, 23 May 2005

This presentation is part of: Biological Chemistry Posters

Biochemical characterization of the amino-terminus of the capsaicin receptor, TRPV1

Christopher Jones, Marta Jimenez, Barry Selinsky, and Joseph Rucker. Villanova University, Villanova, PA

Pain is a mechanism used by multicellular organisms to avoid potentially harmful stimuli in the environment. The receptor TRPV1 is found on the cell surface of pain-sensing neurons and is activated by several different stimuli: noxious heat (>45°), low pH, and vanilloids such as capsaicin, the active ingredient in ‘hot' chili peppers. We have expressed an amino-terminal fragment of TRPV1 in Escherichia coli as a GFP-fusion protein. The expressed protein requires the addition of chaotropic agents for solubilization. We are currently optimizing methods for re-folding the protein. The structural integrity of the fragment is being characterized using several biochemical and spectroscopic methods.

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