Tuesday, 24 May 2005 - 2:45 PM
600

This presentation is part of: College Student Award Symposium sponsored by the Chromatography Forum of Delaware Valley

Effects of Echinostoma caproni larval trematode infection on lipids in the medically important snail Biomphalaria glabrata as determined by HPTLC

Sharon R. Bandstra, Lafayette College, Easton, PA, Bernard Fried, Lafayette College, Easton, PA, and Joseph Sherma, Lafayette College, Easton, PA.

This study examined the effects of larval Echinostoma caproni infection on the neutral lipid and polar lipid content of whole snail bodies of Biomphalaria glabrata infected with cercariae and rediae for six weeks. Uninfected snails were used as controls. As determined by qualitative high performance silica gel thin layer chromatography, the major neutral lipids present in both snail populations were free sterols, free fatty acids, and triacylglycerols, and the major polar lipids were phosphatidylcholine and phosphatidylethanolamine. Quantitative analysis by thin layer chromatography with visible and UV scanning reflectance densitometry showed no significant difference in the concentrations of these lipids in whole bodies of infected snails versus the controls, but the concentration of triacylglycerols in the infected digestive gland-gonad complex was significantly less than that of the uninfected. Studies are in progress to examine the effects of the infection on the hemolymph and shells of infected versus uninfected snails.

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