Tuesday, 24 May 2005 - 2:20 PM
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This presentation is part of: College Student Award Symposium sponsored by the Chromatography Forum of Delaware Valley

Thin Layer Chromatography to Separate Triglyceride Lipase Products

Ang Bian, Kavitha Sompalli, and Peter M. Oelkers. Drexel University, Philadelphia, PA

Most organisms store energy in the form of biologically inert neutral lipids like triglyceride and steryl esters. Our lab focuses on the triglyceride lipases which hydrolyze triglycerides to a combination of fatty acids and glycerides (mono-, di-). Studies conducted on Saccharomyces cerevisiae involved feeding the cells with radiolabelled fatty acid which were incorporated by the organism into more complex lipids such as triglyceride. A phase separation technique was adopted for extraction of the lipids from cell extracts. These cell extracts were resolved by thin layer chromatography technique using silica coated plates and a hexane: ether: acetic acid solvent system. Lipids were separated based on size and hydrophobicity and visualized by iodine staining. The scintillation counter provided the numbers required for quantifying the lipids. Subsequent work will involve use of preparative chromatographic techniques to isolate different triglyceride species which will be used in in vitro assays with purified lipases.

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